Anti-Botulinum neurotoxin type A [CR1]
Product Sizes
1 mg
AB00803-10-3-BT-1MG
About this Product
- SKU:
- AB00803-10-3-BT
- Additional Names:
- BoNT/A; BoNT; Botulinum neurotoxin; Bontoxilysin-A; Bontoxilysin A; BOTOX
- Application:
- Block/Inhibit/Neutralise, Flow Cytometry
- Buffer:
- PBS
- translate.label.attr.clone:
- CR1
- Clonality:
- Recombinant Monoclonal
- Formulation:
- PBS only.
- Host:
- Human
- Immunogen:
- CR1 was produced by a yeast gene diversity library used to increase the affinitiy of scFv AR2 for BoNT/A2, while maintinaing high affinity binding to BoNT/A2. AR2 was generated after multiple rounds of affinity maturation of the humanized C25, which itself was created by CDR-grafting from the parental murine C25. Murine C25 was prepared by constructing a scFv phage display library from mice immunized with recombinant BoNT/A binding domain (Hc).
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Bacteria
- Shipping Conditions:
- Blue Ice
- Specificity:
- CR1 binds to both BoNT/A1 and BoNT/A2 with high affinity (CR1 scfv: KD BoNT/A1 ~ 0.13 nM; KD BoNT/A2 ~ 0.4 nM. CR1 IgG: KD BoNT/A1 ~ 2.5 pM; KD BoNT/A2 ~ 1.7 nM.). CR1 Fab fragment binds to the BoNT/A1 binding domain, at the interface between the N-terminal lectin subdomain (HCN) and the C-terminal trefoil subdomain (HCC) at a discontinuous epitope consisting of B Beta23-B Beta24, B Beta25-B Beta2
- Storage Conditions:
- 2-8[o]C, -20[o]C aliquoted. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00803-10.3-BT
