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Antibodies

Anti-ss/dsDNA [A52]

Product Sizes
50 ug
Was £623.00 £592.00
AB00781-23-0-50UG
About this Product
SKU:
AB00781-23-0
Additional Names:
single-stranded DNA; double-stranded DNA; supercoiled DNA
Application:
Crystallization, ELISA, Radioimmunoassay
Buffer:
PBS with 0.02% Proclin 300.
translate.label.attr.clone:
A52
Clonality:
Recombinant Monoclonal
Extra Details:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Formulation:
PBS with 0.02% Proclin 300.
Host:
Rabbit
Immunogen:
A52 mAb was isolated by producing a hybridoma cell line using spleen cells derived from female unimmunized inbred, autoimmune NZB/NZW F1 mice, which serve as a model for human SLE (systemic lupus erythematosus).
Isotype:
IgG
Purification:
Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Specificity:
A52 binds to both single- and double-stranded DNA (preference for the single-stranded conformation) as well as supercoiled DNA - has a preference for poly(dG) sequences. Poly(dA), poly(dC), poly(rG) and 23S rRNA do not bind to A52. Micelles of cardiolipin (structural analog of the sugar-phosphate backbone of nucleic acids) was shown to be non-inhibitory by ELISA. Both the sugar-phosphate backbone
Storage Conditions:
2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
Supplier:
Absolute Antibody
Type:
Antibody: Recombinant Antibodies