Anti-osteopontin [hu1A12]
Product Sizes
200 ug
AB00750-10-0-200UG
About this Product
- SKU:
- AB00750-10-0
- Additional Names:
- OPN; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; SPP1; Urinary stone protein; uropontin
- Application:
- Block/Inhibit/Neutralise, ELISA, Immunohistochemistry, Surface Plasmon Resonance
- Buffer:
- PBS with 0.02% Proclin 300.
- translate.label.attr.clone:
- hu1A12
- Clonality:
- Recombinant Monoclonal
- Extra Details:
- This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Formulation:
- PBS with 0.02% Proclin 300.
- Host:
- Human
- Immunogen:
- 1A12 antibody was prepared by immunizing BALB/c mice with purified recombinant human OPN. The antibody was humanized by complementarity-determining region grafting the mouse 1A12 CDRs into a human FR template, with some FR residues being substituted for those found to be key in the mouse version to maintain affinity for human OPN. The light and heavy chain expression vectors were co-transfected into COS-7 cells.
- Isotype:
- IgG1
- Purification:
- Purified
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- hu1A12 binds to the epitope (212)NAPSD(216) of the human OPN protein sequence. High levels of OPN expression is associated with highly metastatic cancer cells and has been a target in cancer therapy. OPN has been shown to contribute to the malignancy of breast cancer as it is involved in pathways regulating cell adhesion and invasion, enhancing cell motility produces pro-survival signals by regula
- Storage Conditions:
- 2-8[o]C Aliquot., -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Absolute Antibody
- Type:
- Antibody: Recombinant Antibodies
- Manufacturer's Data Sheet:Ab00750-10.0
