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Antibodies

Rabbit Monoclonal Antibody to His Tag (C-term) (Clone : 25B6E11)

Product Sizes
40 µg
£587.00
45-1089-40UG
About this Product
SKU:
45-1089
translate.label.attr.clone:
25B6E11
Clonality:
Monoclonal
Extra Details:
The rabbit immune system generates antibody diversity and optimizes affinity. technology to generate high affinity and specific monoclonal rabbit antibodies. Anti-His Tag (C-term) Antibody (25B6E11); mAb; Rabbit is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays; helping researchers identify; detect; and purify polyhistidine fusion proteins in bacteria; insect cells; and mammalian cells. This product is not recommended for N-terminal His-fusion protein detection.
Format:
Purified
Host:
Rabbit
Immunogen:
6x His synthetic peptide coupled to KLH
Isotype:
Rabbit IgG
Purification:
Protein A chromatography
Shipping Conditions:
Blue Ice
Storage Conditions:
The antibody is stable in lyophilized form if stored at -20[o]C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8[o]C. For long term storage; aliquot and store at -20[o]C or below. Avoid repeated freezing and thawing cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure-2 : Western blot analysis of His Tag (C-term) Antibody (Clone: 25B6E11) at 0.2 µg/ml on immunoprecipitation from cell lysates containing His fusion protein; 1: His antibody (4 µg) + His tagged fusion protein; 2: Rabbit IgG (4 µg) + His tagged fusion protein; 3: His tagged fusion protein (Input); IRDye 800 Conjugated Goat anti-rabbit IgG (H&L) was used secondry antibody at 0.125 µg/ml.
Figure-3 : Western blot analysis of cross-reactivity of His Tag (C-term) Antibody (Clone: 25B6E11) at 1µg/ml on cell lysates; A: His Tag Antibody (Clone:25B6E11); B: Rabbit IgG (as Negative control); IRDye 800 Conjugated Goat anti-rabbit IgG (H&L) was used as secondry antibody at 0.125 µg/ml.
Figure-3 : Western blot analysis of cross-reactivity of His Tag (C-term) Antibody (Clone: 25B6E11) at 1µg/ml on cell lysates; A: His Tag Antibody (Clone:25B6E11); B: Rabbit IgG (as Negative control); IRDye 800 Conjugated Goat anti-rabbit IgG (H&L) was used as secondry antibody at 0.125 µg/ml.