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Antibodies

Mouse Monoclonal Antibody to Human PD-1 (Clone : PD1.D3)

Product Sizes
40 µg
£486.00
45-1074-40UG
About this Product
SKU:
45-1074
Additional Names:
Programmed cell death protein 1; Protein PD-1; hPD-1; CD279
translate.label.attr.clone:
PD1.D3
Clonality:
Monoclonal
Extra Details:
Programmed cell death protein 1 (PD-1); is cell surface receptor expressing on T cells and pro-B cells. Binding of its two ligands PD-L1 and PD-L2 could result in down-regulation of the immune system by inhibiting the T-cell activation process. Thus; PD-1 is an important immune checkpoint and popular target for therapeutic antibodies against many cancers. Anti-Human PD-1 Antibody (PD1.D3); mAb; Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant human PD-1-Fc .
Format:
Purified
Host:
Mouse
Immunogen:
Recombinant human PD-1-Fc
Isotype:
Mouse IgG1;Kappa
Purification:
Protein A chromatography
Shipping Conditions:
Blue Ice
Storage Conditions:
The antibody is stable in lyophilized form if stored at -20[o]C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8[o]C. For long term storage; aliquot and store at -20[o]C or below. Avoid repeated freezing and thawing cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure-2 : ELISA blocking of human PD-1 antibody (Clone: PD1.D3) against Human PD-L1 recombinant protein binding with Human PD-1 recombinant protein; Coating antigen: PD-1-Fc at 1µg/ml; PD-L1-Fc final concentration: 0.5µg /ml; PD-1 antibody dilution start from 50µg/ml; IC50= 0.33µg/ml
Figure-3 : Flow cytometric analysis of PD-1 Antibody (Clone: PD1.D3) on CHO-K1/PD1 stable cell expressing PD-1 (Red histogram) and CHO negative control cell (Black histogram) at 5 µg/ml; 2.5x10¢µ cells/reaction; iFluor647 conjugated Goat Anti-Mouse IgG used as secondary antibody
Figure-4 : FACS ligand blocking tests of PD-1 Antibody (Clone: PD1.D3) on the binding of human PD-1 cell line with Human PD-L1 (Red histogram);CHO negative control cell (Black histogram); Antibody working concentration: 5 µg/ml; 2.5x10¢µ cells/reaction. Ligand (PD-L1) working concentration: 1 µg/ml; Alexa Fluor 647 Conjugated Affinipure Goat anti-human IgG (H + L) used as secondary antibody
Figure-4 : FACS ligand blocking tests of PD-1 Antibody (Clone: PD1.D3) on the binding of human PD-1 cell line with Human PD-L1 (Red histogram);CHO negative control cell (Black histogram); Antibody working concentration: 5 µg/ml; 2.5x10¢µ cells/reaction. Ligand (PD-L1) working concentration: 1 µg/ml; Alexa Fluor 647 Conjugated Affinipure Goat anti-human IgG (H + L) used as secondary antibody