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Antibodies

Rabbit Polyclonal Antibody to GFP

Product Sizes
40 µg
£474.00
45-1042-40UG
About this Product
SKU:
45-1042
Clonality:
Polyclonal
Extra Details:
Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression; trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants. Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Format:
Purified
Host:
Rabbit
Immunogen:
Purified recombinant full-length GFP protein
Isotype:
Rabbit IgG
Purification:
Immunoaffinity chromatography
Shipping Conditions:
Blue Ice
Storage Conditions:
The antibody is stable in lyophilized form if stored at -20[o]C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8[o]C. For long term storage; aliquot and store at -20[o]C or below. Avoid repeated freezing and thawing cycles.
Supplier:
Abeomics
Type:
Antibodies:Polyclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure-2 : Western blot analysis of GFP Antibody at 1 µg/ml on GFP uv fusion protein (100; 40; 20 & 10 ng); IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
Figure-3 : Western blot analysis of GFP Antibody at 1 µg/ml on YFP fusion protein; IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
Figure-4 : Western blot analysis of GFP Antibody on immunoprecipitates from lysate expressing GFP; 1: Input control material for lysate expressing GFP protein; 2: Negative control with isotype control; 3: Immunprecipitation with GFP Antibody.
Figure-4 : Western blot analysis of GFP Antibody on immunoprecipitates from lysate expressing GFP; 1: Input control material for lysate expressing GFP protein; 2: Negative control with isotype control; 3: Immunprecipitation with GFP Antibody.