Anti-HSP27 Monoclonal Antibody (Clone : 8A7) - ATTO 594
Product Sizes
100 µg
£595.00
42-1185-100UG
About this Product
- SKU:
- 42-1185
- Additional Names:
- HSP27; HSP28
- translate.label.attr.clone:
- 8A7
- Clonality:
- Monoclonal
- Conjugate:
- ATTO 594
- Extra Details:
- HSP25 is the mouse homologue of the human HSP27 protein; a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence; with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers. HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers. The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity; whereas dimers have no chaperone activity. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress; where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus; HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways. Other functions include chaperone activity (as mentioned above); thermo-tolerance in vivo; inhibition of apoptosis; and signal transduction. Specifically; in vitro; it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins; which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex; thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin. And finally; HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth.
- Host:
- Mouse
- Immunogen:
- HSP27 peptide
- Isotype:
- Mouse IgG1 Kappa
- Purification:
- Protein G Purified
- Reactivities:
- Bovine, Human, Mouse, Rat, Sheep
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store the antibody at 4[o]C
- Supplier:
- Abeomics
- Type:
- Antibodies:Monoclonal Antibody
- Manufacturer's Data Sheet:1.jpg
