Polyclonal Antibody to Raf-1
Product Sizes
50 µl
£511.00
35-1757-50UL
100 µl
£581.00
35-1757-100UL
About this Product
- SKU:
- 35-1757
- Additional Names:
- c-RAF|| RAF proto-oncogene serine/threonine-protein kinase
- Clonality:
- Polyclonal
- Extra Details:
- A-Raf; B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338; Tyr341; Thr491; Ser494; Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3;4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445); although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK; respectively (6;7). While A-Raf; B-Raf and c-Raf are similar in sequence and function; differential regulation has been observed (8). Of particular interest; B-Raf contains three consensus Akt phosphorylation sites (Ser364; Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8;9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29; Ser43; Ser289; Ser296; Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to
- Format:
- Purified
- Host:
- Rabbit
- Immunogen:
- Peptide sequence around aa. 641-645(T-S-P-R-L ) derived from Rat Raf-1.
- Isotype:
- Rabbit IgG
- Reactivities:
- Human, Mouse, Rat
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store the antibody at 4[o]C; stable for 6 months. For long-term storage; store at -20[o]C. Avoid repeated freeze and thaw cycles.
- Supplier:
- Abeomics
- Type:
- Antibodies:Polyclonal Antibody
- Manufacturer's Data Sheet:1.jpg
