Polyclonal Antibody to Chk1 (Ab-317)
Product Sizes
50 µl
£511.00
35-1465-50UL
100 µl
£581.00
35-1465-100UL
About this Product
- SKU:
- 35-1465
- Additional Names:
- CHEK1
- Clonality:
- Polyclonal
- Extra Details:
- Chek1 is a protein kinase that inhibits mitotic entry after DNA damage; required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events; such as DNA replication and chromosome segregation; are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%; 40%; and 44% identical to those of the fission yeast Chek1; C. elegans Chek1; and Drosophila 'grapes' (Grp) proteins; respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA; with the most abundant expression in thymus; testis; small intestine; and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation; indicating that Chek1 is modified in response to DNA damage. In vitro; Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation; CDC25C. In response to DNA damage; Chek1 phosphorylates and inhibits CDC25C; thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry Zhang YW; et al. (2005) Mol Cell; 19(5): 607-18. Bhoumik A; et al. (2005) Mol Cell; 18(5): 577-87. Rocha S; et al. (2005) EMBO J. Clarke CA; et al. (2005) Biochem J. Yu X; et al. (2004) Mol Cell Biol; 24(21): 9478-86.
- Format:
- Purified
- Host:
- Rabbit
- Immunogen:
- Peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1.
- Isotype:
- Rabbit IgG
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store the antibody at 4[o]C; stable for 6 months. For long-term storage; store at -20[o]C. Avoid repeated freeze and thaw cycles.
- Supplier:
- Abeomics
- Type:
- Antibodies:Polyclonal Antibody
- Manufacturer's Data Sheet:1.jpg
