Polyclonal Antibody to NF-KB-p100/p52(Phospho-Ser866)
Product Sizes
50 µl
£511.00
35-1014-50UL
100 µl
£581.00
35-1014-100UL
About this Product
- SKU:
- 35-1014
- Additional Names:
- p52|| p105|| H2TF1|| LYT10|| NF-kB2
- Clonality:
- Polyclonal
- Extra Details:
- NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation; immunity; differentiation; cell growth; tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65; RELB; NF-KB1/p105; NF-KB1/p50; REL and NF-KB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors; respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway; I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators; subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway; the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NF-KB2/p100 associated with RelB; inducing its proteolytic processing to NF-KB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NF-KB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3'; located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex; which stimulates the HIV enhancer in synergy with p65. Dobrzanski P.; Ryseck R.P.; Bravo R.EMBO J. 13:4608-4616(1994) Beg A.A.; Baldwin A.S. Jr.Oncogene 9:1487-1492(1994)
- Format:
- Purified
- Host:
- Rabbit
- Immunogen:
- Peptide sequence around phosphorylation site of serine 866(E-D-S(p)-A-Y) derived from Human NF-KB-p100.
- Isotype:
- Rabbit IgG
- Reactivities:
- Human, Mouse, Rat
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store the antibody at 4[o]C; stable for 6 months. For long-term storage; store at -20[o]C. Avoid repeated freeze and thaw cycles.
- Supplier:
- Abeomics
- Type:
- Antibodies:Polyclonal Antibody
- Manufacturer's Data Sheet:1.jpg
