Recombinant Human Myeloperoxidase/MPO (C-10His)
Product Sizes
10 µg
£448.00
32-8958-10UG
50 µg
£493.00
32-8958-50UG
About this Product
- SKU:
- 32-8958
- Extra Details:
- Source: Human Cells. MW :80.3kD. Recombinant Human Myeloperoxidase is produced by our Mammalian expression system and the target gene encoding Ala49-Ser745 is expressed with a 10His tag at the C-terminus. Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide-dependent conversion of chloride; bromide; and iodide to multiple reactive species. Post-translational processing of MPO involves the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked dimer composed of a 60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond between the heavy chains. Alternate splicing generates two additional isoforms of MPO; one with a 32 aa insertion in the light chain; and another with a deletion of the signal sequence and part of the propeptide. Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks. MPO is also associated with a variety of other diseases; and inhibits vasodilation in inflammation by depleting the levels of NO. Serum albuminfunctions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic granules. Upon cellular activation; it is deposited into pathogen-containing phagosomes.
- Shipping Conditions:
- Ambient
- Storage Conditions:
- Lyophilized protein should be stored at -20[o]C; though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7[o]C for 2-7 days. Aliquots of reconstituted samples are stable at -20[o]C for 3 months.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Manufacturer's Data Sheet:download_pdf.php
