CAS9 S. Pyogenes
Product Sizes
50 µg
£1112.00
32-6690-50UG
100 µg
£1640.00
32-6690-100UG
About this Product
- SKU:
- 32-6690
- Additional Names:
- CRISPR-associated endonuclease Cas9/Csn1; SpyCas9; cas9; csn1.
- Extra Details:
- Source: Escherichia Coli.Sterile Filtered colorless solution.Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes; among other bacteria. S. Pyogenes utilizes Cas9 to remember and later probe and cleave foreign DNA; such as invading bacteriophage DNA or plasmid DNA. In case that the DNA substrate is complementary to the guide RNA; Cas9 cleaves the invading DNA. In addition to its original role in bacterial immunity; the Cas9 protein has been heavily employed as a genome engineering tool to induce site-directed double strand breaks in DNA. These disruptions may lead to gene inactivation or the presentation of heterologous genes via non-homologous end joining and homologous recombination respectively in many laboratory model organisms.Recombinant Cas9-NLS produced in E.coli; comprises the entire Cas9 protein sequence (1368 amino acids) joined to a proprietary nuclear localization sequence (NLS) and a 6xHis tag at the C-terminus; having a total of 1414 amino acids. The protein has an apparent molecular weight of 163kDa.
- Purification:
- Greater than 90% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Antibodies:Monoclonal Antibody
- Manufacturer's Data Sheet:download_pdf.php
