FGF 21 Mouse; Sf9
Product Sizes
2 µg
£452.00
32-6356-2UG
10 µg
£528.00
32-6356-10UG
About this Product
- SKU:
- 32-6356
- Additional Names:
- Fibroblast growth factor 21; FGF-21.
- Extra Details:
- Source: Sf9; Baculovirus cells.Sterile Filtered colorless clear solution.The FGFs are a family of more than 20 small (~17–26 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors (FGFRs) 1; 2; or 3. A fourth closely related tyrosine kinase receptor (FGFR4) was able to bind the FGFs but did not lead to a mitogenic response.FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1; -2; -3; and -4; all with intrinsic tyrosine kinase activity and; except for FGFR-4; multiple splice isoforms; and FGFR-5; which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in cells leads to diabetes in mice; which thus implies that proper FGF signaling is required for normal cell function and glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover; FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis.FGF-21 is preferentially expressed in liver; but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes; protects animals from diet-induced obesity when overexpressed in transgenic mice; and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.FGF-21 produced in Sf9 Baculovirus cells is a single; glycosylated polypeptide chain containing 190 amino acids (29-210a.a.) and having a molecular mass of 21.0kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). FGF21 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
- Purification:
- Greater than 90.0% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at 4[o]C if entire vial will be used within 2-4 weeks.Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Antibodies:Monoclonal Antibody
- Manufacturer's Data Sheet:download_pdf.php
