Recombinant HIV-1 TAT; Biotin Labeled
Product Sizes
5 µg
£528.00
32-5590-5UG
About this Product
- SKU:
- 32-5590
- Extra Details:
- Source : Escherichia Coli. HIV-1 TAT Biotin Recombinant- produced in E.coli is a single; non-glycosylated; polypeptide chain containing 86 amino acids encoded by two exons and having chain having a molecular mass of 14kDa and was biotinylated with NHS biotin. Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication and infectivity. The HIV-1 TAT is a molecule of 86 aminoacids encoded by two exons. The product of the first exon is sufficient for the transactivation of the HIV-1 promoter. Biotin molecules can be coupled easily to either antigens (such as avidin; streptavidin; Fc binding proteins like Protein A or G) or antibodies and they can be used in a variety of detection systems. Biotin can be conjugated to many proteins without significantly changing their biological activity. Biotin binds with high affinity to avidin; streptavidin and NeutrAvidin protein. Every single protein can react with several molecules of biotin; each of which; in turn; can bind a molecule of avidin. This greatly increases the sensitivity of many assay procedures. The avidin-biotin interaction is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The bound formation between biotin and avidin is very rapid and; once formed; withstands extremes of pH; organic solvents and other denaturing agents. The avidinbiotin complex can withstand up to 3 M guanidine-HCl. Biotin can be released by 8 M guanidine-HCl at pH 1.5; or by autoclaving.
- Purification:
- Greater than 90.0% as determined by HPLC analysis & SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- HIV-1 TAT biotin recombinant although stable at 4[o]C for 1 week; should be stored below -18[o]C. Please prevent freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Manufacturer's Data Sheet:1.jpg
