Recombinant HepatitisA Virus VP1-P2A (722-830)
Product Sizes
0.5 mg
£1030.00
32-5488-0.5MG
About this Product
- SKU:
- 32-5488
- Extra Details:
- Source : The E.Coli derived 51.2 kDa recombinant protein fused to a GST tag contains the VP1-P2A immunodominant regions; amino acids 722-830. Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein. Nineteen antigenic domains were found within the structural proteins; and 22 were found within the nonstructural proteins; with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant; as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain; located at position 767-842 aa; contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain; located at position 1403-1456 a.a; comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain; located at position 1500-1519 a.a; includes almost the entire P3B and the last domain; located at position 1719-1764 aa; contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. Four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins.
- Purification:
- HAV VP1-P2A protein is >90% pure as determined by 10% PAGE (coomassie staining).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- HAV VP1-P2A protein although stable at 4[o]C for 1 week; should be stored below -18[o]C. Please prevent freeze thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Manufacturer's Data Sheet:1.jpg
