Recombinant Hepatitis C Virus Combined
Product Sizes
0.5 mg
£1030.00
32-5425-0.5MG
About this Product
- SKU:
- 32-5425
- Extra Details:
- Source : The E.Coli derived 70 kDa recombinant protein contains sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then contructed into one recombinant antigen. The protein contains the HCV nucleocapsid; NS3 genotype 1b; NS4 genotype 1b and 1a; and NS5 genotype 1b and 1a immunodominant regions. HCV is a small 50nm; enveloped; single-stranded; positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase; the HCV has an exceptionally high mutation rate; a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2; 3; 5 and 6).
- Purification:
- HCV protein is >95% pure as determined by 10% PAGE (coomassie staining).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- HCV although stable at 4[o]C for 1 week; should be stored below -18[o]C. Please prevent freeze thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Manufacturer's Data Sheet:1.jpg
