Recombinant E.Coli Dnak ATPase Binding Domain
Product Sizes
50 µg
£528.00
32-5304-50UG
About this Product
- SKU:
- 32-5304
- Additional Names:
- HSP-70||HSP70||DnaK||Chaperone protein dnaK||Heat shock protein 70||Heat shock 70 kDa protein||groP||grpF||seg||b0014||JW0013.
- Extra Details:
- Source : Escherichia Coli. Recombinant DnaK Substrate Binding Domain produced in E.Coli is a single; non-glycosylated polypeptide chain containing 384 amino acids and having a molecular mass of 48.1 kDa. DnaK; originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial HSP-70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK(amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain (residues 385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques.
- Purification:
- Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Manufacturer's Data Sheet:1.jpg
