Recombinant Human Ubiquitin Gly76 to Ala76 mutation
Product Sizes
20 µg
£528.00
32-5177-20UG
About this Product
- SKU:
- 32-5177
- Additional Names:
- Ubiquitin||Ribosomal Protein S27a||CEP80||UBA80||UBCEP1||UBCEP80||HUBCEP80||RPS27A||Ubiquitin G76A.
- Extra Details:
- Source : Escherichia Coli. Recombinant human ubiquitin featuring a Gly76 to Ala76 mutation that; by inhibiting the ubiquitin hydrolases; prevents the removal of ubiquitin from protein ubiquitin conjugates. Ubiquitin G76A is expressed in E.coliand purified by ion-exchange chromatography. The conserved 76 amino acid protein ubiquitin (Ub) regulates a host of intracellular processes through its enzymatic conjugation to other cellular proteins.Ubiquitination occurs through sequential steps catalyzed by activating (E1); conjugating (E2); and ligase (E3) enzymes. The final step results in the formation of an isopeptide bond between Ub's C-terminal glycine residue (G76) and a lysine residue of the target protein; although N-terminal ubiquitination is also known.Outcomes of this modification include destabilization of the conjugated protein; altered protein trafficking and functional modulation.After targeting the protein for specific localizations; ubiquitin is released from the substrate by deubiquitinating enzymes.A mutant ubiquitin; having a Gly to Ala substitution at the C-terminus (G76A ubiquitin) supported several downstream reactions of the proteolytic pathway but inhibits the deubiquitination process.As consequence; the Ub derivative becomes irreversibly conjugated to protein; shifting the equilibrium between the bound and unbound form in the direction of conjugation; at the expense of the free form.
- Purification:
- Greater than 95% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store vial at -20[o]C to -80[o]C. When stored at the recommended temperature; this protein is stable for 12 months.Please prevent freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Manufacturer's Data Sheet:1.jpg
