Recombinant Human Trypsin-2
Product Sizes
5 mg
£528.00
32-5140-5MG
About this Product
- SKU:
- 32-5140
- Extra Details:
- Source : Escherichia Coli. Recombinant Human Trypsin-2 is free from any animal and human sources. Recombinant Human Trypsin-2 expressed in E.Coli having a Mw of 24kDa is purified by standard chromatography techniques. Recombinant Human Trypsin-2 is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin-2 is free from protease inhibitors such as PMSF and EDTA. Trypsin (EC3.4.21.4) is part of the serine protease family. Trypsin cleaves lysine and arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester and amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester (BAEE); p-toluenesulfonyl- L-arginine methyl ester (TAME); tosyl-L-arginine methyl ester; N- alpha-benzoyl-L-arginine p-nitroanilide (BAPNA); L-lysyl-p-nitroanilide; and benzoyl-L-tyrosine ethyl ester (BTEE). Serine protease inhibitors that inhibit recombinant trypsin include TLCK (N-p-tosyl-L-lysine chloromethyl ketone); PMSF (phenylmethanesulfonyl fluoride); benzamidine; soybean trypsin inhibitor; and ovomucoid.
- Purification:
- Greater than 90% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Recombinant Human Trypsin although stable at room temp for 1 week; should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Manufacturer's Data Sheet:1.jpg
