Recombinant Human S100 Calcium Binding Protein P
Product Sizes
10 µg
£528.00
32-4761-10UG
About this Product
- SKU:
- 32-4761
- Additional Names:
- Protein S100-P||S100 calcium-binding protein P||S100P||S100E||MIG9.
- Extra Details:
- Source : Escherichia Coli. The Recombinant Human S100P has a molecular mass of 10.4 kDa containing 95 amino acid residues of the human S100P. S100P was originally described as a placental protein of 95 amino acid residues shares about 50% sequence identity with the brain S100 proteins alpha and beta.S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner. S100P in addition to binding Ca2+; also binds Zn2+ and Mg2+. S100P gene is located on chromosome 4p16.S100P is dysregulated in the androgen-independent prostate cancer cell lines LNCaP-R; DU145; and PC3 and may play a role in the etiology of prostate cancer.In ductal hyperplasias; in situ and invasive ductal carcinoma; but not in the normal tissues; S100P overexpression is an early event that might play an important role in the immortalization of human breast epithelial cells in vitro and tumor progression in vivo.In NIH3T3 cells; the expression of S100P led to the presence of S100P in the culture medium; increased cellular proliferation; and enhanced survival following detachment from the culture substrate or after exposure to the chemotherapeutic agent 5-flurouracil. The proliferation and survival effects of S100P expression were duplicated in a time- and concentration-dependent manner by extracellular addition of purified S100P to wild-type NIH3T3 cells and correlated with the activation of Erks and NFB.To determine the mechanisms involved in these effects; we tested the hypothesis that S100P activated RAGE (Receptor for Activated Glycation End-Products). It was found that S100P coimmunoprecipitated with RAGE. Furthermore; the effects of S100P on cell signaling; proliferation and survival were blocked by agents that interfere with RAGE including administration of an amphoterin derived peptide known to antagonize RAGE activation; anti-RAGE antibodies and by expression of a dominant negative RAGE. These data suggest that S100P can act in an autocrine manner via RAGE to stimulate cell proliferation and survival.
- Purification:
- Greater than 95% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store lyophilized protein at -20[o]C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4[o]C for a limited period of time; it does not show any change after two weeks at 4[o]C.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Manufacturer's Data Sheet:1.jpg
