Recombinant Bacterial Outer Membrane Protein-A
Product Sizes
200 µg
£858.00
32-4373-200UG
About this Product
- SKU:
- 32-4373
- Additional Names:
- Outer Membrane Protein-A||OmpA.
- Extra Details:
- Source : Escherichia Coli. The OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A.salmonicida; Shigella dysenteriae and E.coli.OmpA's major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity; as well as a role in conjugation and bacteriophage binding.Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease.Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein.The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E.coli BL21(DE3). The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein.Recombinant A-protein was compared by biochemical; immunological and molecular methods with the A-protein isolated from atypical A.salmonicida bacterial cells by the glycine and the membrane extraction methods.
- Purification:
- Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Lyophilized Bacterial Outer Membrane Protein-A although stable at room temperature for 3 weeks; should be stored desiccated below -18[o]C. Upon C between 2-7 days and for future use reconstituted OmpA should be stored at 4 below -18[o]C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Manufacturer's Data Sheet:1.jpg
