Recombinant Mouse Lipopolysaccarid Binding Protein
Product Sizes
5 µg
£686.00
32-4099-5UG
About this Product
- SKU:
- 32-4099
- Additional Names:
- Lipopolysaccharide-binding protein||LBP||Ly88.
- Extra Details:
- Source : Chinese Hamster Ovarian Cells (CHO). LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off. Up to 2µg/ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (2 x 106/ml). Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein (BPI); LBP binds LPS and interacts with the CD14 receptor; most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins; including BPI; plasma cholesteryl ester transfer protein (CETP); and phospholipid transfer protein (PLTP). The LBP gene is found on chromosome 20; directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles; to phospholipid bilayers; and to a binding site on soluble CD14 (sCD14). sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate; and then yielding it to an HDL particle; therefore acting as a soluble 'shuttle' for an insoluble lipid.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks; should be stored desiccated below -18[o]C. Upon reconstitution LBP should be stored at 4[o]C between 2-7 days and for future use below -18[o]C.Please prevent freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Manufacturer's Data Sheet:1.jpg
