Recombinant Human Ku P70/P80
Product Sizes
20 µg
£528.00
32-4083-20UG
About this Product
- SKU:
- 32-4083
- Additional Names:
- ATP-dependent DNA helicase 2 subunit 1||ATP-dependent DNA helicase II 70 kDa subunit||Lupus Ku autoantigen protein p70||Ku70||70 kDa subunit of Ku antigen||Thyroid-lupus autoantigen||TLAA||CTC box-binding factor 75 kDa subunit||CTCBF||CTC75||DNA-repa
- Extra Details:
- Source : Sf9 insect cells. KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated; polypeptide chain having a molecular mass of 70;638 Dalton for the p70 subunit and 83;528 Dalton for the p80 subunit. Ku (P70/P80) is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Ku antigen consists of two polypeptides (70 kDa and 80 kDa; the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair; V(D)J recombination; telomere maintenance; and the regulation of specific gene transcription. Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome (up to 20% of patients) but have also been detectable in about 20 % of patients with primary pulmonary hypertension (as a complication of overlap); in 5-10 % of SLE and MCTD patients; and in up to 20 % of patients with primary Sjgren's syndrome.In IIF using HEp-2 cells; Ku-positive sera yield a dense; fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent.
- Purification:
- Greater than 95% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. Avoid multiple freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
- Manufacturer's Data Sheet:1.jpg
