EIF3J Recombinant Protein
Product Sizes
10 µg
£528.00
32-3734-10UG
About this Product
- SKU:
- 32-3734
- Additional Names:
- Eukaryotic translation initiation factor 3 subunit J||Eukaryotic translation initiation factor 3 subunit 1||eIF-3-alpha||eIF3 p35||EIF3J||EIF3S1||eIF3-alpha.
- Extra Details:
- Source : Escherichia Coli. EIF3J Human Recombinant produced in E.coli is a single; non-glycosylated polypeptide chain containing 210 amino acids (70-258) and having a molecular mass of 24kDa (Molecular weight on SDS-PAGE will appear higher).EIF3J is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Eukaryotic translation initiation factor 3 subunit J (EIF3J) is a member of the EIF-3 subunit J family. EIF3 has a crucial role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to produce the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA access channel of the 40S subunit; setting EIF3J directly in the ribosomal decoding center. In addition; EIF3J interacts with eIF1A and reduces 40S subunit affinity for mRNA. An elevated affinity for mRNA is restored upon enlistment of initiator tRNA; despite the fact EIF3J remains in the mRNA-binding cleft in the presence of tRNA.
- Purification:
- Greater than 90.0% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Manufacturer's Data Sheet:1.jpg
