PKACa2/RIIa2 Recombinant Protein
Product Sizes
3 µg
£562.00
32-3084-3UG
About this Product
- SKU:
- 32-3084
- Additional Names:
- Protein Kinase A holoenzyme type II alpha||PKACa2-RIIa2.
- Extra Details:
- Source : Escherichia Coli. Inactive holoenzyme consisting of one dimeric regulatory subunit type II alpha and two monomeric catalytic subunits (cAMP-free). Protein Kinase A is purified by proprietary chromatographic techniques. The protein kinase A holoenzyme is a heterotetramer composed of two types of subunits: Catalytic and Regulatory. The Catalyticsubunit contains the enzyme's active site. It also contains a domain that binds ATP and a domain that binds the regulatory subunit. The Regulatory subunit consists oftwo molecules which bind one another in an anti-parallel orientation to form a homodimer; for type I subunits- this binding is covalent via disulfide bonds. This subunit also has has two domains that bind cyclic AMP; a domain that interacts with a catalytic subunit; and an 'auto-inhibitory' domain that serves as a substrate or pseudosubstrate for the catalytic subunit. Regulatory subunits may also have biologic activity distinct from their role in modulating catalytic subunit activity. Regulatory subunits exist in two major forms; RI and RII; with each form having two subtypes designated alpha and beta. Each of the four isotypes of the regulatory subunit is encoded by a different gene. In addition; three isotypes of the catalytic subunit have been identified (alpha; beta and gamma). The different isotypes tend to have different distributions within cells and among tissues. Type I enzymes inhabit cytoplasmic; soluble fractions of the cell; whereas type II enzymes tend to associate with cellular membranes.
- Purification:
- Greater than 95% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- PKA should be stored at 4[o]C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20[o]C. Avoid multiple freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Manufacturer's Data Sheet:1.jpg
