UBE2M Recombinant Protein
Product Sizes
20 µg
£528.00
32-2897-20UG
About this Product
- SKU:
- 32-2897
- Additional Names:
- NEDD8-conjugating enzyme Ubc12||Ubiquitin-conjugating enzyme E2 M||NEDD8 protein ligase||NEDD8 carrier protein||UBC12||hUbc12||UBC-RS2.
- Extra Details:
- Source : Escherichia Coli. UBE2M Human Recombinant produced E. coli is a single polypeptide chain containing 207 amino acids (1-183) and having a molecular mass of 23.5kDa.UBE2M is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. UbcH12 is functional in in vitro NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APP-BP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UbcH12 in driving cells through the S to M checkpoint. It was demonstrated to be the E2 responsible for the NEDDylation of the Cul-1 component of the SCF (TRCP) complex which is important as the E3-ligase in the ubiquitinylation of I?B?. NEDDylation of Cul-1 is essential for conjugation and processing of NF-kB p105 by SCF following phosphorylation of the complex. A dominant negative form of UbcH12; previously demonstrated to sequester NEDD8 and inhibit its conjugation; inhibits both conjugation and processing of p105; which is alleviated by wild-type UbcH12.
- Purification:
- Greater than 95% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Manufacturer's Data Sheet:1.jpg
