TOP1 Recombinant Protein
Product Sizes
20 µg
£528.00
32-2855-20UG
About this Product
- SKU:
- 32-2855
- Additional Names:
- DNA topoisomerase 1||EC 5.99.1.2||DNA topoisomerase I||TOP1||Scl-70.
- Extra Details:
- Source : Sf9 insect cells. DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated; polypeptide chain having a molecular mass of 91;576 Dalton (excluding glycosilation) 102 kDa total mass. The TOP1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kDa molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kDa molecular weight from which the Scl-70 antigen has derived its name.
- Purification:
- Greater than 95% as determined by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at 4[o]C if entire vial will be used within 2-4 weeks. Store; frozen at -20[o]C for longer periods of time. Avoid multiple freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
- Manufacturer's Data Sheet:1.jpg
