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NTH Recombinant Protein

Product Sizes
10 µg
£528.00
32-2617-10UG
About this Product
SKU:
32-2617
Additional Names:
DNA-(apurinic or apyrimidinic site) lyase||b1633||JW1625.
Extra Details:
Source : Escherichia Coli. NTH E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single; non-glycosylated; polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 25.7kDa. The NTH is purified by proprietary chromatographic techniques. Endonuclease III (nth) is a DNA repair enzyme which has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases numerous damaged pyrimidines from DNA by cleaving the N-glycosidic bond and leaving an AP (apurinic/apyrimidinic) site. This AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination; thus leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Purification:
Greater than 85.0% as determined by SDS-PAGE.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 4[o]C if entire vial will be used within 2-4 weeks.Store; frozen at -20[o]C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Supplier:
Abeomics
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules
  • Manufacturer's Data Sheet:1.jpg