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ISG15 (human) (Rhodamine 110)

Product Sizes
50 µg
£683.00
32-190039-50UG
About this Product
SKU:
32-190039
Additional Names:
ISG15 Ubiquitin-like Modifier; G1P2; IFI15; IP17; UCRP
Extra Details:
Source: E. coli ; MW ~18kDa ; ISG15 (Interferon Stimulated Gene; 15kDa) is a ubiquitin-like modifier which initiates innate immune response by activating RIG-I signaling; stimulating NK-cell proliferation; inhibiting viral budding and acting as an IFNγ-inducing cytokine. ISG15 contains two tandem ubiquitin homology domains and is cross-reactive with α-Ubiquitin antibodies. Conjugation of ISG15 to a substrate protein occurs through a ubiquitin like cascade via an E1 (UBE1L); E2 (UbcH8/UBE2L6) and E3 (not yet discovered.) Deconjugation normally occurs via UBP43 (USP18); however there are several viral proteases that are able to hydrolyze ISG15 conjugates in order to evade immune response. These include the OTU-containing protease of Crimean-Congo Hemorrhagic Fever nairovirus and the Papain-Like Protease (PLPro) of the SARS coronavirus.
Purification:
>=97% (LCMS)
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Short Term Storage -80[o]C Long Term Storage -80[o]C Handling Advice Aliquot to avoid freeze/thaw cycles. Use/Stability Stable for at least 1 year after receipt when stored at -80[o]C.
Supplier:
Abeomics
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules
  • Manufacturer's Data Sheet:1.jpg
Figure 2: Robustness of Rhodamine 110 vs. 7-amino-4- methylcoumarin (AMC) substrates. Fluorescent substrates (500nM ISG15-Rh110/ ISG15-AMC) were incubated with and without 100pM PLpro (SARS Papain-Like-Protease) in a 384 well plate (n=16); and progress curves were normalized to the maximum fluorescence signal to produce “% reaction progress”. The Z’ value; a statistical parameter widely used in the evaluation of screening assays; was calculated at each time-point.
Figure 2: Robustness of Rhodamine 110 vs. 7-amino-4- methylcoumarin (AMC) substrates. Fluorescent substrates (500nM ISG15-Rh110/ ISG15-AMC) were incubated with and without 100pM PLpro (SARS Papain-Like-Protease) in a 384 well plate (n=16); and progress curves were normalized to the maximum fluorescence signal to produce “% reaction progress”. The Z’ value; a statistical parameter widely used in the evaluation of screening assays; was calculated at each time-point.