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CHST5 Human

Product Sizes
2 µg
£452.00
32-13684-2UG
10 µg
£528.00
32-13684-10UG
About this Product
SKU:
32-13684
Additional Names:
Carbohydrate sulfotransferase 5; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4; GST4; Intestinal N-acetylglucosamine-6-O-sulfotransferase; I-GlcNAc6ST; Intestinal GlcNAc-6-sulfotransferase; mIGn6ST; N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST-3; Gn6st-3; Chst5; G
Extra Details:
Source:Sf9; Baculovirus cells.Physical Appearance:Sterile filtered colorless solution.Biological ActivitySpecific activity is greater than 10;000 pmol/min/ug; and is defined as the amount of enzyme that sulfate from PAPS to Nacetyl-D-glucosamine per minute at pH 7.5; at 37°C.Carbohydrate Sulfotransferase 5 (CHST5) is a Golgi-embedded enzyme that is found in B cells; T cells and intestinal epithelium and is also mediates sulfation of keratan in cornea. CHST5 is a sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine residues of keratan. CHST5 works on the non-reducing terminal GlcNAc of short andlong carbohydrate substrates that have poly-N-acetyllactosamine structures.CHST5 Human produced in Sf9 Baculovirus cells is a single; glycosylated polypeptide chain containing 380 amino acids (27-395 a.a.) and having a molecular mass of 42.9kDa.CHST5 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Format:
CHST5 protein solution (0.25mg/ml) containing 20% glycerol and Phosphate-Buffered Saline (pH 7.4).
Purification:
Greater than 90.0% as determined by SDS-PAGE.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 4[o]C if entire vial will be used within 2-4 weeks.Store; frozen at -20[o]C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Supplier:
Abeomics
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules