SPARC Human
Product Sizes
50 µg
£665.00
32-13448-50UG
200 µg
£823.00
32-13448-200UG
About this Product
- SKU:
- 32-13448
- Additional Names:
- Osteonectin; ON; Basement-membrane protein 40; BM-40; SPARC; Secreted Protein acidic and Rich in Cysteine.
- Extra Details:
- Source: Escherichia Coli.Sterile Filtered White lyophilized (freeze-dried) powder.SPARC; an acronym for “secreted protein; acidic and rich in cysteineâ€; is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid; 43 kDa protein contains a 17 aa signal sequence; an N-terminal acidic region that binds calcium; a follistatin domain containing Kazal-like sequences; and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts; capillary endothelial cells; platelets and macrophages; especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects; but generally inhibits adhesion; spreading and proliferation; and promotes collagen matrix formation. For endothelial cells; SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone; where it promotes osteoblast differentiation and inhibits adipogenesis.Secreted Protein Acidic & Rich in Cysteine Human Recombinant produced in E.Coli is a single; non- glycosylated polypeptide chain containing 286 amino acids and having a molecular mass of 32.7kDa.SPARC is purified by proprietary chromatographic techniques.
- Purification:
- Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Lyophilized SPARC although stable at room temperature for 3 weeks; should be stored desiccated below -18[o]C. Upon reconstitution Secreted Protein Acidic & Rich in Cysteine should be stored at 4[o]C between 2-7 days and for future use below -18[o]C.Please prevent freeze-thaw cycles.
- Supplier:
- Abeomics
- Type:
- Antibodies:Monoclonal Antibody
- Manufacturer's Data Sheet:download_pdf.php
