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Antibodies

Anti-alpha-Tubulin Monoclonal Antibody (Clone: YOL1/34)

Product Sizes
0.1 mg
£541.00
30-2930-0.1MG
About this Product
SKU:
30-2930
Additional Names:
TUBA
translate.label.attr.clone:
YOL1/34
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
The rat monoclonal antibody YOL1/34 recognizes an epitope of alpha-tubulin localized between amino acids 414-422. It has higher affinity for fixed microtubules than for native ones.
Format:
Purified
Immunogen:
Yeast tubulin
Isotype:
Rat IgG2a
Purification:
Purified by protein-G affinity chromatography.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
Figure 2: Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in sample 6 μg/ml; DAR FITC; left picture) vs. Hoechst 34580 nuclear staining (right picture).
Figure 3: Western blotting analysis of human alpha-tubulin using rat monoclonal antibody YOL1/34 on lysates of Caenorhabditis elegans; Arabidopsis thaliana; Trypanosoma brucei; Drosophila melanogaster; human HeLa cell line; and porcine brain; all under reducing conditions. Each lane contains 20 μg of total protein. Nitrocellulose membrane was probed with 2 µg/ml of rat anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated anti-rat secondary antibody.
Figure 4: Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-alpha-tubulin (YOL1/34) purified antibody (concentration in sample 8 μg/ml; GAM APC).
Figure 4: Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-alpha-tubulin (YOL1/34) purified antibody (concentration in sample 8 μg/ml; GAM APC).
Figure 5: Separation of human monocytes stained using anti-alpha-tubulin (YOL1/34) purified antibody (concentration in sample 8 μg/ml; GAM APC; red-filled) from monocytes stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 8 μg/ml; same as alpha-tubulin purified; GAM APC; black-dashed) in flow cytometry analysis (intracellular staining).