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Antibodies

APC conjugated Anti-Human IL-17A Mab (Clone: 9F9)

Product Sizes
100 tests
£649.00
30-2870AC-100TESTS
About this Product
SKU:
30-2870AC
Additional Names:
Interleukin 17; interleukin 17A
translate.label.attr.clone:
9F9
Clonality:
Monoclonal
Conjugate:
APC
Extra Details:
Specificity: The mouse monoclonal antibody 9F9 recognizes human interleukin 17A (IL-17A; secreted or intracellular). Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules; chemokines; antimicrobial peptides; and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells; and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis; psoriasis; multiple sclerosis; and another inflammatory diseases; including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13; enhancing retention of B cells within the germinal centers; B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT); and it is involved in epithelial barrier formation upon injury.
Host:
Mouse
Immunogen:
mammalian-derived human IL-17-IgG fusion protein; boost with recombinant human IL-17A
Isotype:
Mouse IgG1 kappa
Purification:
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Protect from prolonged exposure to light. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2: Flow cytometry multicolor surface staining pattern of PHA stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and intracellular staining using anti-human IL-17A (9F9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Figure 3: Separation of human CD17A positive CD3 positive lymphocytes (red-filled) from CD17A negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Figure 3: Separation of human CD17A positive CD3 positive lymphocytes (red-filled) from CD17A negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-17A (9F9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).