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Antibodies

Anti-Perforin FITC MAb (Clone: dG9)

Product Sizes
100 Tests
£581.00
30-2839-100TESTS
About this Product
SKU:
30-2839
Additional Names:
PRF1; P1; PFP; HPLH2;Perforin 1
translate.label.attr.clone:
dG9
Clonality:
Monoclonal
Conjugate:
FITC
Extra Details:
Specificity: The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin; a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells. Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis; defects of its gene can cause severe disorders.
Host:
Mouse
Immunogen:
Purified granules from human YT lymphoma cell line
Isotype:
Mouse IgG2b kappa
Purification:
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Reactivities:
Bovine, Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Protect from prolonged exposure to light. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2: Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD56 (LT56) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human Perforin (dG9) FITC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Figure 3: Separation of human Perforin positive CD56 positive lymphocytes (red-filled) from Perforin negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Perforin (dG9) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
Figure 3: Separation of human Perforin positive CD56 positive lymphocytes (red-filled) from Perforin negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Perforin (dG9) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).