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Antibodies

Alexa Fluor 488 Conjugated Anti-Human IL-2 MAb (Clone: 35C3)

Product Sizes
100 tests
£649.00
30-2836AF488-100TESTS
About this Product
SKU:
30-2836AF488
Additional Names:
Interleukin 2
translate.label.attr.clone:
35C3
Clonality:
Monoclonal
Extra Details:
IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However; IL-2 also stimulates growth and differentiation of B cells; NK cells; monocytes and other cell types; such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein. Specificity: The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular).
Host:
Mouse
Immunogen:
Recombinant human IL-2
Isotype:
Mouse IgG2b kappa
Purification:
Purified antibody is conjugated with Alexa Fluor 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Protect from prolonged exposure to light. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2: Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood) and intracellular staining using anti-human IL-2 (35C3) Alexa Fluor 488 antibody (10 µl reagent / 100 µl of peripheral whole blood).
Figure 3: Separation of human IL-2 positive CD3 positive lymphocytes (red-filled) from IL-2 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Alexa Fluor 488 antibody (10 µl reagent / 100 µl of peripheral whole blood).
Figure 3: Separation of human IL-2 positive CD3 positive lymphocytes (red-filled) from IL-2 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Alexa Fluor 488 antibody (10 µl reagent / 100 µl of peripheral whole blood).