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Antibodies

Anti-Human TCR Cbeta1 FITC (Clone : JOVI.1)

Product Sizes
100 tests
£581.00
30-2489-100TESTS
About this Product
SKU:
30-2489
Additional Names:
TCRCB1; TRBC1;T cell receptor beta constant 1
translate.label.attr.clone:
JOVI.1
Clonality:
Monoclonal
Conjugate:
FITC
Extra Details:
Alpha-beta T cell receptors (TCRs) are antigen specific receptors; which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs); that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK; that phosphorylates the ITAM motifs of CD3gamma; CD3delta; CD3epsilon and CD3zeta; enabling the recruitment of ZAP70. In turn; ZAP70 phosphorylates LAT; which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways; the calcium signaling; the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways; leading to the mobilization of transcription factors; that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted; non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.Specificity : The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
Immunogen:
Thymus; spleen; and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Isotype:
Mouse IgG2a kappa
Purification:
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store in the dark at 2-8[o]C. Do not freeze. Avoid prolonged exposure to light.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2 : Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Cbeta1 (JOVI.1) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Figure 3 :Separation of human TCR Cbeta1 positive T cells (red-filled) from TCR Cbeta1 negative lymphocytes(black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).
Figure 3 :Separation of human TCR Cbeta1 positive T cells (red-filled) from TCR Cbeta1 negative lymphocytes(black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).