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Antibodies

Anti-5-bromodeoxyuridine (BrdU) Monoclonal Antibody (Clone:Bu20a)-APC Conjugated

Product Sizes
100 (T100) tests (T)
£605.00
30-1486-AC-100
About this Product
SKU:
30-1486-AC
Additional Names:
5-bromodeoxyuridine
translate.label.attr.clone:
Bu20a
Clonality:
Monoclonal
Conjugate:
APC
Extra Details:
Specificity: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU. Description: Bromodeoxyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates; tissue sections or suspensions using an antibody specific for the BrdU.
Host:
Mouse
Immunogen:
Bromodeoxyuridine conjugated to BSA
Isotype:
Mouse IgG1
Purification:
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Protect from prolonged exposure to light. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2: Flow cytometry multicolor intracellular staining pattern of K562 cells cultivated with BrdU stained using anti-BrdU (Bu20a) APC antibody (concentration in sample 8μg/ml) and Propidium iodide.
Figure 3: Separation of K562 cells cultivated with BrdU stained using anti-BrdU (Bu20a) APC antibody (concentration in sample 8μg/ml; red-filled) from K562 cells cultivated with BrdU stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 8μg/ml; same as BrdU APC concentration; black-dashed) in flow cytometry analysis (intracellular staining).
Figure 3: Separation of K562 cells cultivated with BrdU stained using anti-BrdU (Bu20a) APC antibody (concentration in sample 8μg/ml; red-filled) from K562 cells cultivated with BrdU stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 8μg/ml; same as BrdU APC concentration; black-dashed) in flow cytometry analysis (intracellular staining).