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Antibodies

Anti-GFP / Green Fluorescent Protein Polyclonal Antibody

Product Sizes
0.1 mg
£551.00
30-1330-0.1MG
About this Product
SKU:
30-1330
Clonality:
Polyclonal
Extra Details:
Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria; emiting green light (lambda=509 nm) when excited (excitation by Blue or UV light; absorption peak lambda=395 nm). GFP is a useful tool in cell biology research; as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts; including microbes; invertebrates; vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP; since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression; enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP; chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein; Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
Format:
Purified
Host:
Rabbit
Immunogen:
EGFP; a native full-length protein
Isotype:
Rabbit IgG
Purification:
Purified from rabbit serum by affinity chromatography
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Polyclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2: Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris; pH 7.5; 100 mM NaCl; 0.5% Triton X-100; inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated using a polyclonal anti-GFP antibody (lane 2) or a pre-immune rabbit serum (lane 3). Immunoprecipitates together w
Figure 2: Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris; pH 7.5; 100 mM NaCl; 0.5% Triton X-100; inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated using a polyclonal anti-GFP antibody (lane 2) or a pre-immune rabbit serum (lane 3). Immunoprecipitates together w