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Antibodies

Anti-CD22 Monoclonal Antibody (Clone:MEM-01)

Product Sizes
0.1 mg
£532.00
30-1166-0.1MG
About this Product
SKU:
30-1166
Additional Names:
CD22||SIGLEC2
translate.label.attr.clone:
MEM-01
Clonality:
Monoclonal
Extra Details:
CD22; also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2;6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs); thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Format:
Purified
Host:
Mouse
Immunogen:
Raji Burkitt's lymphoma cell line
Isotype:
Mouse IgG1
Purification:
Purified by protein-A affinity chromatography
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2: Separation of human CD22 positive lymphocytes (red-filled) from human CD22 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0;6 μg/ml; GAM APC).
Figure 3: Anti-Hu CD22 Purified (clone MEM-01) reactivity pattern in WB application under non-reducing conditions. The reactivity of MEM-01 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji; Ramos; Daudi; and Jurkat cell lines; mixed and heated (100°C; 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.
Figure 3: Anti-Hu CD22 Purified (clone MEM-01) reactivity pattern in WB application under non-reducing conditions. The reactivity of MEM-01 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji; Ramos; Daudi; and Jurkat cell lines; mixed and heated (100°C; 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.