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Antibodies

Anti-Cytokeratin (Pan-reactive) Monoclonal Antibody (Clone:C-11)

Product Sizes
0.1 mg
£532.00
30-1043-0.1MG
About this Product
SKU:
30-1043
translate.label.attr.clone:
C-11
Clonality:
Monoclonal
Extra Details:
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides; molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Format:
Purified
Host:
Mouse
Immunogen:
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Isotype:
Mouse IgG1
Purification:
Purified by protein-A affinity chromatography
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure-2: Immunocytochemistry staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 (30-1043; diluted 1:400); detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).
Figure-3: Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-cytokeratins followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for cytokeratins at approximately 45-55 kDa.
Figure-3: Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-cytokeratins followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for cytokeratins at approximately 45-55 kDa.