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Antibodies

Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free

Product Sizes
0.1 mg
£532.00
30-1028-0.1MG
About this Product
SKU:
30-1028
Additional Names:
CD34
translate.label.attr.clone:
QBEnd-10
Clonality:
Monoclonal
Extra Details:
CD34 is a highly glycosylated monomeric 111-115 kDa surface protein; which is present on many stem cell populations. It is a well established stem cell marker; though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion; differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Host:
Mouse
Immunogen:
Human endothelial vesicles
Isotype:
Mouse IgG1
Purification:
Purified by protein-A affinity chromatography
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Do not freeze.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure-2: Flow cytometry multicolor surface staining of human peripheral blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0;6 μg/ml; GAM APC; red-filled) and anti-human CD45 (MEM-28) Pacific Blue antibody (10 μl reagent / 100 μl of peripheral whole blood).
Figure-3: Separation of human CD45dim CD34 positive stem cells (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0;6 μg/ml; GAM APC).
Figure-4: Western blotting analysis of human CD34 using mouse monoclonal antibody QBEnd-10 on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody QBEnd-10 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.
Figure-4: Western blotting analysis of human CD34 using mouse monoclonal antibody QBEnd-10 on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody QBEnd-10 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.
Figure-5: Flow cytometry analysis (surface staining) of CD34 in human peripheral blood with anti-CD34 (QBEnd-10) azide free.