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Antibodies

anti-IL-33 (human); mAb (IL33305B)

Product Sizes
50 µg
£512.00
29-1011-50UG
100 µg
£609.00
29-1011-100UG
About this Product
SKU:
29-1011
Additional Names:
Interleukin-33; IL-1F11; NF-HEV
translate.label.attr.clone:
IL33305B
Extra Details:
Recognizes human IL-33. Does not cross-react with mouse IL-33.Interleukin-33 (IL-33; HF-NEV; IL-1F11); a member of the IL-1 family of cytokines; is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88; IRAK; IRAK4; and TRAF6; followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1); p38(MAPK14); and JNK. The ability of IL-33 to target numerous immune cell types; like Th2-like cells; mast cells; and B1 cells; and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases; such as arthritis; asthma; atopic allergy & anaphylaxis; cardiovascular disease/atherosclerosis; nervous system diseases; and sepsis
Host:
Mouse
Immunogen:
Recombinant human IL-33.
Isotype:
Mouse IgG2a;k
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Stable for at least 1 year after receipt when stored at -20[o]C.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2: Inhibition of binding of recombinant human IL-33 (hIL-33) to human ST2 (hST2) in vitro using anti-IL-33 (human); mAb (IL33305B). An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add 2 ug/ml of IL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2;000) to an enzyme; 4) after adding the TMB solution; incubate at RT in the dark for 20 minutes. Immediately read the plate at
Figure 3: Immunoprecipitation of recombinant hIL-33 proteins using anti-IL-33 (human); mAb (IL33305B). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE; electroblotted; and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.
Figure 4: Western blot analysis of human IL-33 using anti-IL-33 (human); mAb (IL33305B) at 1:2;000 dilution.1. Recombinant human IL-33 (His tagged). 2. Recombinant human IL-33 (FLAG®-tagged). 3. Recombinant mouse IL-33 (FLAG®-tagged). 4. Other protein (His tagged; negative control).
Figure 4: Western blot analysis of human IL-33 using anti-IL-33 (human); mAb (IL33305B) at 1:2;000 dilution.1. Recombinant human IL-33 (His tagged). 2. Recombinant human IL-33 (FLAG®-tagged). 3. Recombinant mouse IL-33 (FLAG®-tagged). 4. Other protein (His tagged; negative control).