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SARS-CoV-2 Spike S1 D614G Stable Trimer Recombinant His Protein

Product Sizes
50µg
£595.00
21-1014-50UG
About this Product
SKU:
21-1014
Extra Details:
Full-length soluble SARS-CoV-2 Spike protein (aa 16-1213) including a foldon trimerization motif; mutated Furin recognition site (R682S; R685S) and two stabilizing mutations (K986P and V987P) is fused at the C-terminus to a His-tag. Source: Expressed in HEK-293 cells. Reconstitution: Reconstitute with 250µl endotoxin-free water. Concentration: 0.2mg/ml after reconstitution. Background: The spike S1 protein of SARS-CoV-2 attaches the virus to its cellular receptor; angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S1 mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses; as well as protective immunity.
Purification:
>90% by SDS-PAGE.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped on ice packs. Upon arrival; Store at -20[o]C. Do not freeze-thaw multiple times. For maximum product recovery after thawing; centrifuge the vial before opening the cap.
Supplier:
Abeomics
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules
Figure 1: Full-length trimeric SARS-CoV-2 Spike (Cat. No. 21-1014) binds to its receptor; Human ACE2 Protein; FLAG Tag (Cat. No. 32-190010); with high affinity. Method: SARS-CoV-2 Spike S1 D614G Stable Trimer Recombinant His Protein (Cat. No. 21-1014) or SARS-CoV-2 Spike Protein S1 RBD recombinant His protein (Cat. No. 32-190055) are coated at 50nM overnight at 4°C. Human ACE2 Protein; FLAG Tag (Cat. No. 32-190010) is added (starting at a concentration of 8000 ng/ml with a twofold serial dilution) during one hour at RT and the interaction is then detected using an anti-Flag (HRP).
Figure 2: SDS-PAGE: Coomassie staining under non-reducing conditions; showing one clear band at ~190kDa and confirming purity of the protein.
Figure 3: SARS-CoV-2 Spike S1 D614G Stable Trimer Recombinant His Protein (Cat. No. 21-1014) migrates by Size Exclusion Chromatography (SEC) as a single peak. Method: SARS-CoV-2 Spike S1 D614G Stable Trimer Recombinant His Protein has been injected in a SEC column ENrich SEC 650 10 x 300 mm (Bio-Rad).