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FLOW IntraCellular Staining Kit (IC FLOW)

Product Sizes
1 kit
£276.00
17-1012-1KIT
About this Product
SKU:
17-1012
Extra Details:
The Intracellular FLOW Staining Kit allows detection antibodies to access the intracellular protein in Flowcytometry analysis. This kit is specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and to increase fluorescence signaling. First; live cells can be fixed with the Fixation buffer; which cross-links the protein. Second; the 1X Permeabilization Buffer creates holes in the membrane; allowing the antibodies enter into the cells effectively. Washing steps and antibody dilutions are done using 1X Permeabilization Buffer. The final cell resuspension prior to analysis is done using The Staining Buffer. Permeabilization Buffer is supplied as a 10X solution. Prior to use; this should be diluted 10 fold in distilled water. (1ml of Permeabilization Buffer with 9 ml of distilled water). 10X Permeabilization buffer has natural tendency to precipitate; however; its function is not affected. 1X solution can be filtered.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C
Supplier:
Abeomics
Type:
Assay Kits:Staining & Imaging
Figure-1: Intracellular Staining of PBMC. 0.5 µg of antibody was used. Red: Human TLR3 PE (10-3004PE). Green: Isotype control (mouse IgG1PE) ABEOMICS was used. Intracellular FLOW kit from ABEOMICS was used.