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Assay Kits & ELISAs

Annexin V-FITC Apoptosis Detection Kit

Product Sizes
100 Tests
£386.00
17-1002-100TESTS
About this Product
SKU:
17-1002
Extra Details:
Apoptosis or programmed cell death is an evolutionary conserved; intrinsic program of cell death that occurs in various physiological and pathological situations. It is a key regulator of physiological growth control and regulation of tissue homeostasis. Apoptosis is considered a vital component of various processes including normal cell turnover; proper development and functioning of the immune system; hormone-dependent atrophy; embryonic development and chemical-induced cell death. The term apoptosis (a-po-toe-sis) was first used in a now-classic paper by Kerr; Wyllie; and Currie in 1972 to describe a morphologically distinct form of cell death; although certain components of the apoptosis concept had been explicitly described many years previously. Apoptosis is characterized by specific morphological changes of the dying cells; namely; loss of membrane asymmetry; cytoskeleton remodeling; plasma membrane blebbing; loss of the mitochondrial membrane potential; caspase activation; chromatin condensation; and DNA fragmentation. During apoptosis; the asymmetric distribution of phospholipids of the plasma membrane gets lost and phosphatidylserine (PS) is translocated to the outer leaflet of the plasma membrane. There; PS acts as one major “eat me” signal that ensures efficient recognition and uptake of apoptotic cells by phagocytes. Once on the cell surface; PS can be easily detected by staining with a fluorescent conjugate of Annexin V; a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS; and binds to cells with exposed PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store all kit components at 4[o]C.
Supplier:
Abeomics
Type:
Assay Kits:Detection
  • Manufacturer's Data Sheet:1.png
Fig.2: Adherent RAW cells were treated with 0.05 µg/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Celltase and stained with the Annexin V FITC Apoptosis Detection Kit.
Fig.2: Adherent RAW cells were treated with 0.05 µg/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Celltase and stained with the Annexin V FITC Apoptosis Detection Kit.