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Celltase™ Cell Detachment Solution

Product Sizes
100 ml
£177.00
17-1001-100ML
500 ml
£430.00
17-1001-500ML
About this Product
SKU:
17-1001
Extra Details:
Celltase is a ready-to-use cell detachment solution of made up of proteolytic and collagenolytic enzymes. Celltase is a direct replacement for Trypsin solution and is very useful for the routine cell disassociation from standard tissue culture plastic-ware and other adhesion coated polymers. Celltase is devoid of any mammalian and bacterial derived products. It performs efficiently in detaching cells for the analysis of cell surface markers; routine cell passage; proliferation assays; production scale-up of bioreactors; various bioassays and flow cytometry among others. Primary cells tested for Celltase application include fibroblasts; keratinocytes; hepatocytes; mouse bone marrow cells; hESCs; macrophages and PBMCs; neuronal stem cells. Celltase is shown to be effective on A431; HeLa; 293 cells; L929 cells; 3T3; A549; adherent CHO and BHK cells; HT1080 fibrosarcoma cells; Sf9 insect cells; Vero; COS; NT2; MG63; M24. As compared to Trypsin; CelltaseTM is gentle on cells and it auto-inhibits at 37°C.
Shipping Conditions:
Blue Ice
Storage Conditions:
2 months at 2-8[o]C. 12 month if frozen at -20[o]C. For long term or intermittent use the product may be aliquoted and frozen if necessary.
Supplier:
Abeomics
Type:
Buffers & General Consumables:Others
Fig. 2: A431 cells were detached with Celltase @ RT with 5 and 15 minutes of incubation time. Cells were washed; harvested and stained using anti-Epcam antibody (Catalogue no: 10-7515). Anti mouse PE was used as secondary antibody. Cells were analyzed by flow cytometry and both the files were overlapped. Expression of Epcam antigen on the cell surface after Celltase treatment was similar in both the time points. Celltase is gentle on surface antigens and cell membrane unlike Trypsin.
Fig. 2: A431 cells were detached with Celltase @ RT with 5 and 15 minutes of incubation time. Cells were washed; harvested and stained using anti-Epcam antibody (Catalogue no: 10-7515). Anti mouse PE was used as secondary antibody. Cells were analyzed by flow cytometry and both the files were overlapped. Expression of Epcam antigen on the cell surface after Celltase treatment was similar in both the time points. Celltase is gentle on surface antigens and cell membrane unlike Trypsin.