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Molecular Biology

CpG ODN (C274); TLR9 ligand (Class C)

Product Sizes
0.1mg
£243.00
15-1022-0.1MG
1mg
£551.00
15-1022-1MG
About this Product
SKU:
15-1022
Extra Details:
Sequence: 5'-tCGtCGaaCGttCGagatgat-3' (Class C ODN C274: Bases are phosphorothioate.) Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motif are equivalent to bacterial DNA in the immunostimulatory activity; which can induce innate immunity via Toll-like receptor 9 (TLR9) in mammals. There are three major classes of CpG ODNs; and each ODN class exhibits different stimulatory effects on immune cell activation. Class A ODNs are potent inducers of IFN-alpha that leads to the plasmacytoid dendritc cell (pDC) maturation. Class B ODNs are relatively weak inducers of type I IFNs but strong stimulators of human B cells and monocyte maturation. Class C ODNs combine elements of both Classes A and B ODNs; which can induce IFN-alpha in pDC and activation of B cells.
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Upon receipt; store at -20[o]C (Stable for at least 6 months). Avoid frequent freeze/thaw cycles.
Supplier:
Abeomics
Type:
Molecular Biology Reagents:Nucleic Acids
Fig-2: Induction of mouse TLR9 activity by CpG ODN C274.
Fig-3: Abeomics' CpG ODNs did not show any TLR2 agonist activity. TLR2/NF-kB Leeporter™ HEK293 cells (14-127ACL) were treated with various CpG ODNs at 100 ug/ml as well as a positive TLR2 agonist; Pam3CSK4; at 10 ng/ml for 16 h; and luciferase activity was then analyzed.
Fig-4: Abeomics' CpG ODNs did not show any TLR4 agonist activity. TLR4/IL-8 Leeporter™ HeLa cells (14-124ACL) were treated with various CpG ODNs at 100 ug/ml as well as a positive TLR4 agonist; LPS; at 10 ng/ml for 16 h; and luciferase activity was then analyzed.
Fig-4: Abeomics' CpG ODNs did not show any TLR4 agonist activity. TLR4/IL-8 Leeporter™ HeLa cells (14-124ACL) were treated with various CpG ODNs at 100 ug/ml as well as a positive TLR4 agonist; LPS; at 10 ng/ml for 16 h; and luciferase activity was then analyzed.