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TLR7/HEK293 Stable Cell Line

Product Sizes
1 Vial
£2747.00
14-536ACL-1VIAL
About this Product
SKU:
14-536ACL
Extra Details:
TLR7/HEK293 Stable Cell Line is a stably transfected HEK293 cell line which expresses human Toll-like receptor 7 (TLR7; also designated as CD287). TLR7 mediates immune responses by recognition of single-stranded RNA of viruses and involves in the pathogenesis of rheumatic diseases such as systemic lupus erythematosus. TLR7 also responds to synthetic small molecules such as imiquimod and resiquimod (aka R-848) which act as immunomodulators. Note that TLR7 in the TLR7/HEK293 stable cell line contains the N-terminal HA tag (Figure 2); which does not interfere with TLR7 activity as confirmed by functional assay (Figure 1). Sequence data: Human TLR7 (accession number NP_057646) MVFPMWTLKRQILILFNIILISKLLGARWFPKTLPCDVTLDVPK NHVIVDCTDKHLTEIPGGIPTNTTNLTLTINHIPDISPASFHRLDHLVEIDFRCNCVP IPLGSKNNMCIKRLQIKPRSFSGLTYLKSLYLDGNQLLEIPQGLPPSLQLLSLEANNI FSIRKENLTELANIEILYLGQNCYYRNPCYVSYSIEKDAFLNLTKLKVLSLKDNNVTA VPTVLPSTLTELYLYNNMIAKIQEDDFNNLNQLQILDLSGNCPRCYNAPFPCAPCKNN SPLQIPVNAFDALTELKVLRLHSNSLQHVPPRWFKNINKLQELDLSQNFLAKEIGDAK FLHFLPSLIQLDLSFNFELQVYRASMNLSQAFSSLKSLKILRIRGYVFKELKSFNLSP LHNLQNLEVLDLGTNFIKIANLSMFKQFKRLKVIDLSVNKISPSGDSSEVGFCSNART SVESYEPQVLEQLHYFRYDKYARSCRFKNKEASFMSVNESCYKYGQTLDLSKNSIFFV KSSDFQHLSFLKCLNLSGNLISQTLNGSEFQPLAELRYLDFSNNRLDLLHSTAFEELH KLEVLDISSNSHYFQSEGITHMLNFTKNLKVLQKLMMNDNDISSSTSRTMESESLRTL EFRGNHLDVLWREGDNRYLQLFKNLLKLEELDISKNSLSFLPSGVFDGMPPNLKNLSL AKNGLKSFSWKKLQCLKNLETLDLSHNQLTTVPERLSNCSRSLKNLILKNNQIRSLTK YFLQDAFQLRYLDLSSNKIQMIQKTSFPENVLNNLKMLLLHHNRFLCTCDAVWFVWWV NHTEVTIPYLATDVTCVGPGAHKGQSVISLDLYTCELDLTNLILFSLSISVSLFLMVM MTASHLYFWDVWYIYHFCKAKIKGYQRLISPDCCYDAFIVYDTKDPAVTEWVLAELVA KLEDPREKHFNLCLEERDWLPGQPVLENLSQSIQLSKKTVFVMTDKYAKTENFKIAFY LSHQRLMDEKVDVIILIFLEKPFQKSKFLQLRKRLCGSSVLEWPTNPQAHPYFWQCLK NALATDNHVAYSQVFKETV
Shipping Conditions:
Blue Ice
Storage Conditions:
Immediately upon receipt; store in liquid nitrogen.
Supplier:
Abeomics
Type:
Cells:Cell Lines
Fig-1: Functional analysis of the TLR7/HEK293 cell line. TLR7/HEK293 cells as well as parental HEK293 cells were transfected with NF-kB/Renilla luciferase reporter plasmid for 16 h. Cells were stimulated with different doses of Resiquimod; R-848 (Abeomics; Cat. #15-1016) for 16 h followed by luciferase assay using Luciferase Assay Reagent (Abeomics; Cat. #17-1101).
Fig-2: Western blot analysis of TLR7 expression in the TLR7/HEK293 cell line. Cell lysates were analyzed by SDS-PAGE followed by Western blotting using anti-HA antibody. Note that TLR7 in the TLR7/HEK293 stable cell line contains the N-terminal HA tag. Control; parental HEK293 cell lysate; TLR7; TLR7/HEK293 cell lysate.