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Antibodies

Anti-CD33 antibody(DM77); Rabbit mAb

Product Sizes
100 µg
£801.00
12-9251-100UG
About this Product
SKU:
12-9251
Additional Names:
CD33;SIGLEC3;gp67;SIGLEC-3
translate.label.attr.clone:
DM77
Clonality:
Monoclonal
Extra Details:
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2;3- and more avidly alpha-2;6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
Host:
Rabbit
Purification:
Purified from cell culture supernatant by affinity chromatography
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C for 12 months (Avoid repeated freezing and thawing)
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD33 (B) were surface stained with Rabbit anti-CD33 monoclonal antibody 1µg/ml (clone: DM77) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD33 monoclonal antibody (clone: DM77) on Expi 293 cell line transfected with human CD33. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Flow cytometry analysis of antigen binding of rabbit anti-human CD33 mAb. (A) Anti-human CD33 mAb does not bind to 293T cells that do not express CD33. (B) A clear peak shift of anti-human CD33 mAb was seen compared to the control when incubated with CD33-expressing THP-1 cells; indicating strong binding of anti-human CD33 mAb to CD33. Antibodies were incubated at 10 µg/mL.
Figure 4. Flow cytometry analysis of antigen binding of rabbit anti-human CD33 mAb. (A) Anti-human CD33 mAb does not bind to 293T cells that do not express CD33. (B) A clear peak shift of anti-human CD33 mAb was seen compared to the control when incubated with CD33-expressing THP-1 cells; indicating strong binding of anti-human CD33 mAb to CD33. Antibodies were incubated at 10 µg/mL.