Anti-Zika (ZIKV) E Protein-Purified in vivo GOLD Functional Grade
Product Sizes
50 mg
£2285.00
12-8298-50MG
100 mg
£3058.00
12-8298-100MG
About this Product
- SKU:
- 12-8298
- Additional Names:
- ZIKV E; Envelope protein
- translate.label.attr.clone:
- ZV67
- Clonality:
- Monoclonal
- Extra Details:
- Specificity: Clone ZV-67 binds to the Zika virus envelope (E) protein at domain III (DIII; LR). 1 Antigen Distribution: The Envelope (E) protein expressed on the Zika Virus Background: Zika virus (ZIKV) infection during pregnancy is a global public health problem 1; linked causally to severe fetal abnormalities 2. Prophylactic antibodies may prove useful in treating pregnant patients or for designing epitope-specific vaccines 1. The mouse monoclonal antibody (MAb) ZV-67 specifically targets ZIKV and neutralizes infection of the American; Asian; and African strains to varying degrees 1. ZIKV is a mosquito-transmitted flavivirus that encodes a single polyprotein with an ~11 kb positive-sense RNA open reading frame 1. The polyprotein is cleaved into seven non-structural (NS) proteins and three structural proteins (capsid (C); pre-membrane (prM); and envelope (E)). C forms a nucleocapsid. prM complexes with E to facilitate folding and prevent premature fusion to host membranes. E is responsible for viral assembly; attachment; entry; and fusion 1;3 and is a major target of neutralizing antibody research 3. Mature ZIKV virions incorporate 180 copies each of the E and M proteins 4;5. E is divided into three domains; DI; DII; and DIII 3. DI is a central ?-barrel; DII is an extended dimerization domain; and DIII is an immunoglobulin-like segment. The lateral ridge of DIII is targeted by the ZV-67 MAb 1. ZV-67 was generated by priming a lethal mouse model with ZIKV (MR-766 and H/PF/2013) and DIII domain. ZV-67 is of the IgG2c isotype and has been shown to neutralize the MR-766; Uganda 1947; Dakar 41519; and Senegal 1982 African strains as well as the American Paraiba 2015; Brazil strain. It has no cross-reactivity with Japanese Encephalitis or Dengue. Analysis of antibody contact residues by X-ray crystallography shows that ZV-67 binds to the heavy chain complementarity determining region of DIII. A total of 21 residues are contacted by ZV-67; representing four discrete secondary structure elements of the A-strand; B-C; D-E; and F-G loops.
- Host:
- Mouse
- Purification:
- Purity: >=95% monomer by analytical SEC Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a mu
- Reactivities:
- Mouse
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- This antibody may be stored sterile as received at 2-8[o]C for up to one month. For longer term storage; aseptically aliquot in working volumes without diluting and store at
- Supplier:
- Abeomics
- Type:
- Antibodies:In Vivo Antibodies
- Manufacturer's Data Sheet:download_pdf.php
